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Bile Salt Powder

Bile salts are steroidal detergents, which together with lipids/fats/cholesterol form mixed micelles in the intestine to enable fat digestion and absorption through the intestinal wall. They are biosynthesized from cholesterol in the liver and stored in the gall bladder.

Bile derivatives like bile salts, bile salt mixtures and pure substances like sodium deoxycholate and sodium taurocholate are used as selective agents mainly to inhibit gram-positive organisms, non-intestinal organisms and spore formers while permitting the growth of gram-negative enterics. Bile is secreted through the bile duct into the intestine when food passes from the stomach to the duodenum. Most of the bile salts secreted into the upper region of the small intestine are absorbed along with dietary lipids at the lower end of the small intestine. They are separated from the dietary lipid and returned (more than 85%) to the liver for re-circulation. This movement of bile salts is termed as enterohepatic circulation. Resistance to bile salts is considered an important parameter for selecting probiotic strains. A concentration of 0.15-0.3 % of bile salt has been recommended as a suitable concentration for selecting probiotic bacteria for human use.

Use of Bile Salt Powder:

Bile salt powder of Titan Biotech Ltd. is developed specifically for microbiological culture media applications. It is a mixture of two salts of sodium cholate and sodium deoxycholate. It is totally different from Ox bile extract in many ways. It is usually prepared for MacConkey Agar and Broth, Brilliant Green Bile Broth and E.E Broth. It provides constant media with light colour w/o interfering the dyes and clarity. It does not allow growing faecal Staphylococci and Streptococci.

BILE SALT 3-alpha,7-alpha-dihydroxy-5-beta-cholan-24-oic acid
Formula: C24H39NaO5 (NaC); C24H39NaO4 (NaD)
Molecular weight: 430.6 (NaC); 414.6 (NaD)

Solubility:

However, bile conjugation increases the aqueous solubility of bile salts under physiological conditions. The chemical structures of free and conjugated cholic acid (taurocholate and glycolate). All primary bile acids appear to have three features in common:

  • They are the major end-products of cholesterol metabolism
  • They are secreted into the bile largely in a conjugated form and
  • these conjugates are membrane-impermeable, water-soluble, amphiphilic molecules having a powerful ability to transform lamellar arrays of lipids into mixed micelles.

  • Bile Salt Mixture (STD) TBL Powder
  • Bile Salt (STD) TBL Powder
  • Bile Powder (OX Bile Desiccated)

BILE SALT MIXTURE (STD), TBL POWDER

CODE- 1235 (Culture Media Ingredient)

Bile salt mixture is a standard Titan Biotech Ltd. powder which is developed specifically as a microbiological culture media ingredient. Bile derivatives like bile salts, bile salt mixtures and pure substances like sodium deoxycholate and sodium taurocholate are used as selective agents mainly to inhibit gram-positive organisms, non-intestinal organisms and spore formers while permitting the growth of gram-negative enterics. Bile secreted in the small intestine reduces the survival of bacteria by destroying their cell membranes, the major components of which are lipids and fatty acids; these modifications may affect not only the cell permeability and viability, but also the interactions between the membrane and the environment (Succi et al., 2005). Resistance to bile salts is considered an important parameter for selecting probiotic strains. A concentration of 0.15-0.3 % of bile salt has been recommended as a suitable concentration for selecting probiotic bacteria for human use (Goldin BR and Gorbach 1992). Bile Salts has the evolution stages, some of which are intermediates in the biosynthesis of C24 bile acids, those are described for all the chief vertebrate groups.

C24 bile salts may be considered as hydroxylated chenodeoxycholic acid. Conjugated bile salts have powerful detergent properties, which are important in stabilizing the super saturated state of bile and in promoting fat digestion and absorption.

[C24H39Na04; Molecular Weight = 414.6 (NaD); CAS no. 302-95-4]
[C24H39Na05; Molecular Weight = 430.6 (NaC) CAS no. 361-09-1]

Use:

It is a mixture of two salts of sodium cholate and sodium deoxycholate. It is usually added as ingredient in microbiological culture media, such as in MacConkey Agar and Broth, Brilliant Green Bile Broth and E.E Broth. It provides constant media with light colour w/o interfering the dyes and clarity. It does not allow growing faecal Staphylococci and Streptococci.

Solubility:

However, bile conjugation increases the aqueous solubility of bile salts under physiological conditions. The chemical structures of free and conjugated cholic acid (taurocholate and glycolate). The solubility of bile salt mixture is freely soluble in water, and is sparingly in ethanol, whereas, this is insoluble in choloroform, acetone and diethyl ether.

BILE SALT (STD) TBL POWDER

CODE- 1234 (Culture Media Ingredient)

Bile salt is a standard Titan Biotech Ltd. powder is developed specifically as a microbiological culture media ingredient. Bile salts are composed of the salts of four different kinds of free bile acids (cholic, deoxycholic, chenodeoxycholic, and lithocholic acids); each of these acids may in turn combine with glycine or taurine to form more complex acids and salts. Bile salts and acids can be synthesized from cholesterol or extracted from the bloodstream by the liver. Bile secreted in the small intestine reduces the survival of bacteria by destroying their cell membranes, the major components of which are lipids and fatty acids; these modifications may affect not only the cell permeability and viability, but also the interactions between the membrane and the environment (Succi et al., 2005). Resistance to bile salts is considered an important parameter for selecting probiotic strains. A concentration of 0.15-0.3 % of bile salt has been recommended as a suitable concentration for selecting probiotic bacteria for human use (Goldin BR and Gorbach 1992).

It is used as culture media ingredients, used as selective agents mainly to inhibit gram - positive organisms, non-intestinal organisms and psore formers while permitting the growth of gram - negative entrics. It is usually prepared for MacConkey Agar and Broth, Brilliant Green Bile Broth and E.E Broth. It provides constant media with light colour w/o interfering the dyes and clarity. It does not allow growing faecal Staphylococci and Streptococci.

Use:

It is a mixture of two salts of sodium cholate and sodium deoxycholate. It is usually added as ingredient in microbiological culture media, such as in MacConkey Agar and Broth, Brilliant Green Bile Broth and E.E Broth. It provides constant media with light colour w/o interfering the dyes and clarity. It does not allow growing faecal Staphylococci and Streptococci.

Solubility:

However, bile conjugation increases the aqueous solubility of bile salts under physiological conditions. The chemical structure of bile salt is a free and conjugated cholic acid (taurocholate and glycolate). The solubility of bile salt mixture is freely soluble in water, and in alcohol, whereas, this is insoluble in choloroform, acetone and diethyl ether and has clear solution with no precipitation.

BILE POWDER (OX BILE DESICCATED)

CODE- TBF 007

This Bile powder (Ox bile desiccated) permanently bound to insoluble cellulose as a dietary supplement and may increase the absorption of vitamin E. Bile is steroidal detergents, which together with lipids/fats/cholesterol form mixed micelles in the intestine to enable fat digestion and absorption through the intestinal wall. They are biosynthesized from cholesterol in the liver and stored in the gall bladder.

Use:

Bile powder (Ox Bile Desiccated) is developed specifically for pharma applications. This desiccated form of bile helps in absorption of vitamin E with typically low and variable levels. Vitamin E is absorbed from the lumen of the small intestine into the enterocytes by passive diffusion. Prior to its absorption, vitamin E is emulsified together with dietary lipids. Esterified forms of vitamin E, alpha-locopheryl acetate and succinate, undergo hydrolysis via esterases secreted by the pancreas. The micelles containing vitamin E are absorbed at the brush border of the intestinal mucosa in the enterocytes. Excess solid drug was dispersed in taurodeoxycholate or mixed micelle solutions prepared with fixed mole ratios of taurocholate, taurodeoxycholate, taurochenodeoxycholate, glycodeoxycholate, glycocholate, and glycochenodeoxycholate with egg phosphatidylcholine.

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