Instruction

DISSOLVING THE MEDIA
Weigh the required amount of dehydrated culture media and place it in an intact and clean container at least two times larger than the final volume of media to be produced. Use distilled or deionised water which matcheswith the specification of purified water of IP/USP. Add part of water and other permissible heat stable ingredients (if any) and swirl the container thoroughly. Addremaining water to make up the required volume.Heat the medium to boiling with frequent agitation to prevent overheating of the medium.

pH ADJUSTMENT

The pH value of the dehydrated culture media shall produce the equivalent value at 25 C as mentioned on the lable. pH adjustment (if the required pH is different should be carried out with In r 0.1N Hydrochloric acid or Sodium hydroxide solution).

STERILIZATION

Generally, sterilization is done at 15 psi (corresponds to 121 C) for 15 minutes using autoclave. Volumes larger than 2 liters may require additional acutocalving time to achieve proper sterilization.

DISPENSING OF THE MEDIA

Cool the agar based media to 47 2 C and aseptically add heat labile or heat sensitive enrichments or supplements sterilized separately by suitable method. Sterile broths may be cooled to room temperatures before addition of the supplements. Dispense the medium in to the sterile petri dishes, tubes etc as per the requirements and immediately plug orrecover. Allow the agar based media to solidify for 1-2 hrs.

STORAGE OF PREPARED MEDIA

If the prepared media is not to be used with in 24 hrs then is should at low temperature (2-8 C) in moisture proof containers. Petri plates should be inverted and the caps of the tubes be tighten firmly before storage.

DISPOSAL OF MEDIA

All samples and cultures should be handled carefully and should not be disposed without autoclaving at 15 psi for 30 minutes.