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AGAR AGAR POWDER (as per IP) Bacteriological Grade

Agar is phycocolloid extract of Red -purple marine algae (Class Rhodophyceae) including Gelidium, Gracilaria and Pterocladia. Agar is a vegetable Gelatin and one with high gelling properties which is used in microbiological culture media. It has characteristics of good clarity, controlled gelling strength and melting temperature. Good diffusion capacity absence of toxic inhibitions and free from minerals. Provide moderate flow and lighting with the supplement of Iron, Magnesium and Trace elements. High levels of phosphates or nitrates will inhibit the growth of these algae. Agar is used clinical, bacterial and yeast transformation studies and molecular biology studies, bacteriological agar is generally used 1 - 2% for microbiological culture media preparations 0.5% for motility and anaerobes and microaerophiles require 0.1% maximum. Agar is a gel at room temperature, remaining firm at temperature as high as 65°C. Agar melts at approximately 85 - 88°C, a different temperature from that at which it solidifies, 32 - 40°C. This property is known as hysteresis. Agar is generally resistant to shear forces; however, different agars may have different gel strengths or degrees of stiffness.

PHYSICAL PARAMETERS

S.L. No.

PRODUCT PARAMETERS

SPECIFICATION

1.

Appearance

Off white to creamish colour free flowing powder

2.

Solubility (2% soln. at 90°C)

Soluble in hot water, near to clear

3.

Clarity (1% soln. at 121ºC.)*

Near to clear, No ppt.

* -After Autoclave 

CHEMICAL PARAMETERS

S.L. No.

PRODUCT PARAMETERS

SPECIFICATION

1.

pH  (2% soln. at 25ºC)

6.0-7.0

2.

Loss on drying (at 105ºC)

NMT-10.0%

3.

Acid Insoluble Ash

NMT- 0.5%

4.

Gelling Temp.

38-40ºC

5.

Melting Temp.

85-88ºC

6.

Total Ash

NMT-4.0%

7.

Gel Strength (1.5% Gel)

NLT- 800 gm/cm2

8.

Heavy Metals (Pb)

NMT- 20ppm

     MICROBIAL PARAMETERS

S.L. No.

PRODUCT PARAMETERS

SPECIFICATION

1.

Total viable count

NMT- 10,000cfu/gm

2.

Escherichia coli

Negative

3.

Salmonella spp.

Negative

4.

Staphylococcus aureus

Negative

5.

Yeast & molds

100/gm

Std Packing   : 25.0 Kg, HDPE Drum

Best Before   : 36 Months

Storage         : Hygroscopic in nature, store below 25ºC and protect direct sunlight.

 

 
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Agar Agar Powder
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