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BILE SALT (Equivalent to Bile Salt No.3)

Description:
Bile salt is a product by Titan Biotech Ltd., which is developed specifically as a microbiological culture media ingredient. It consists of pure substances of sodium deoxycholate and sodium taurocholate, those are used as selective agents mainly to inhibit gram-positive organisms, non-intestinal organisms and spore formers while permitting the growth of gram-negative enterics.

Use:
• It is usually added as ingredient in microbiological culture media, such as in MacConkey Agar and Broth, SS Agar, Violet Red Bile Agar, Brilliant Green Bile Broth and E.E Broth.
• Bile salt increases the selectivity of the medium by inhibiting gram positive microbes.
• This bile salts entered in the growth pattern and altered fermentation of lactose.
• It does not allow growing faecal Staphylococci and Streptococci.

PHYSICAL PARAMETERS

S.L. No.

PRODUCT PARAMETERS

SPECIFICATION

1.

Appearance

White colour free flowing powder, no pungent smell.

2.

Solubility (2% soln. at 25oC)

Soluble in distilled water.

3.

Clarity (2% Soln. at 121 ºC.)*

Clear solution. No ppt.


* - After Autoclave

CHEMICAL PARAMETERS

S.L. No.

PRODUCT PARAMETERS

SPECIFICATION

1.

pH  (2% Soln. at 25 ºC)

6.5 - 8.0

2.

Loss on drying ( at 105 ºC)

NMT - 5.0%



MICROBIAL PARAMETERS

S.L. No.

PRODUCT PARAMETERS

SPECIFICATION

1.

Total viable count

NMT- 10,000cfu/gm

2.

Escherichia coli

Negative

3.

Salmonella spp.

Negative

4.

Staphylococcus aureus

Negative

5.

Yeast & molds

100/gm



GROWTH PROMOTION TEST

Cultural Characteristic observed in MacConkey agar with 0.15% Bile salt mixture after incubation at 35-37°C for 18-24 hours.

S.L. No.

MICROORGANISMS

ATCC

GROWTH

RECOVERY

1.

Staphylococcus aureus

25923

Inhibited

--------

2.

Escherichia coli

25922

Good-Luxuriant /Pink bile ppt.

>= 50%

3.

Enterococcus faecalis

29212

Good-Luxuriant, Pink

>= 50%

4.

Salmonella typhimurium

14028

Good-Luxuriant, colourless

>= 50%



Best Before : 36 Months

Storage : Hygroscopic by nature, store the material below 25 ºC, protect from direct sunlight.

* We certify the above product is having least or no risk of TSE / BSE.
 
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