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Home » Products » Biotechnology & Fermentation » BILE SALT MIXTURE (STD), TBL POWDER Culture Media Ingredient

BILE SALT MIXTURE (STD), TBL POWDER Culture Media Ingredient

Composed of these two salts of bile:
[C24H39Na04; Molecular Weight = 414.6 (NaD); CAS no. 302-95-4]
[C24H39Na05; Molecular Weight = 430.6 (NaC) CAS no. 361-09-1]

Use:
• It is usually added as ingredient in microbiological culture media, such as in MacConkey Agar and Broth, SS Agar, Violet Red Bile Agar, Brilliant Green Bile Broth and E.E Broth.
• Bile salt mixture increases the selectivity of the medium to Enterobacteriaceae by inhibiting gram positive bacteria.
• This mixture of bile salts entered in the growth pattern and altered fermentation of lactose.
• It does not allow growing faecal Staphylococci and Streptococci.

PHYSICAL PARAMETERS

S.L. No.

PRODUCT PARAMETERS

SPECIFICATION

1.

Appearance

Creamish to white colour free flowing powder, no pungent smell.

2.

Solubility (2% soln. at 25oC)

Completely soluble in distilled water, clear.

3.

Clarity (2% Soln. at 121 ºC.)*

Absolute clear solution. No ppt.


* - After Autoclave

CHEMICAL PARAMETERS

S.L. No.

PRODUCT PARAMETERS

SPECIFICATION

1.

pH  (2% Soln. at 25 ºC)

7.0 - 8.0

2.

Loss on drying ( at 105 ºC)

NMT - 5.0%

3.

Assay (as Sodium Cholate & Sodium Deoxycholate )

NLT - 98.0%



MICROBIAL PARAMETERS

S.L. No.

PRODUCT PARAMETERS

SPECIFICATION

1.

Total viable count

NMT- 5,000cfu/gm

2.

Escherichia coli

Negative

3.

Salmonella spp.

Negative

4.

Staphylococcus aureus

Negative

5.

Yeast & molds

50/gm



GROWTH PROMOTION TEST
Cultural Characteristic observed in MacConkey agar with 0.15% Bile Salt (STD) TBL after incubation at 35-37°C for 18-24 hours.

S.L. No.

MICROORGANISMS

ATCC

INOCULUMS

(CFU)

GROWTH

RECOVERY

1.

Staphylococcus aureus

25923

--------

Inhibited

---------

2.

Escherichia coli

25922

50-100

Good-Luxuriant

>= 50%

3.

Enterococcus faecalis

29212

50-100

Good-Luxuriant

>= 50%

4.

Streptococcus pyogenes

19615

50 - 100

Good-Luxuriant

>= 50%



Best Before : 36 Months

Storage : Hygroscopic by nature, store the material below 25 ºC, protect from direct sunlight.

* We certify the above product is having least or no risk of TSE / BSE.
 
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