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BILE SALT POWDER(Bacteriological Grade) Culture Media Ingredient

Description:
Bile salt constitutes pure substances, sodium conjugates of glycotaurocholate, and those are used as selective agents mainly to inhibit gram-positive organisms, non-intestinal organisms and spore formers while permitting the growth of gram-negative enterics. Bile salt inhibits Enterococcus and Staphylococcus species.

Use:
Bile salt powder of Titan Biotech Ltd. is developed specifically for biotechnology and culture media applications.
• Bile salt transformation and degradation is of high ecological relevance and also essential for the biotechnological production of drugs.
• Bile salt entered in the growth pattern and altered fermentation of lactose.

PHYSICAL PARAMETERS

S.L. No.

PRODUCT PARAMETERS

SPECIFICATION

1.

Appearance

Greenish to yellowish colour free flowing powder, no pungent smell.

2.

Solubility (2% soln. at 25oC)

 Soluble in distilled water and alcohol.

3.

Clarity (2% Soln. at 121 ºC.)*

Clear solution. No ppt.

* - After Autoclave

CHEMICAL PARAMETERS

S.L. No.

PRODUCT PARAMETERS

SPECIFICATION

1.

pH  (2% Soln. at 25 ºC)

6.5 - 8.0

2.

Loss on drying ( at 105 ºC)

NMT - 5.0%

3.

Total Ash

NMT - 20.0%

4.

Cholic Acid (as Sodium Cholate)

NLT - 45.0%



MICROBIAL PARAMETERS

S.L. No.

PRODUCT PARAMETERS

SPECIFICATION

1.

Total viable count

NMT- 10,000cfu/gm

2.

Escherichia coli

Negative

3.

Salmonella spp.

Negative

4.

Staphylococcus aureus

Negative

5.

Yeast & molds

100/gm



GROWTH PROMOTION TEST
Cultural Characteristic observed in MacConkey agar with 0.5% Bile salt Powder after incubation at 35-37°C for 18-24 hours.

S.L. No.

MICROORGANISMS

ATCC

GROWTH

RECOVERY

1.

Staphylococcus aureus

25923

Inhibited

-----------

2.

Escherichia coli

25922

Luxuriant / bile ppt.

>= 50%

3.

Enterococcus faecalis

29212

Fair-Good

>= 50%

4.

Salmonella typhimurium

14028

Luxuriant

>= 50%



Best Before : 36 Months

Storage : Hygroscopic by nature, store the material below 25 ºC, protect from direct sunlight

* We certify the above product is having least or no risk of TSE / BSE.
 
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