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BILE SALT (STD) TBL POWDER Culture Media Ingredient

Description:
Bile salt (STD) TBL powder is developed by Titan Biotech Ltd. specifically for microbiological culture media, which is more purified and light in colour. Bile salts are bile acids compounded with a cation, usually sodium. The salts of taurocholic acid and glycocholic acid represent bile salts. This Sodium glycotaurocholate are used as selective agent mainly to inhibit the gram positive organisms and non-intestinal organisms.

Use:
• It is usually added as ingredient in microbiological culture media, such as in MacConkey Agar and Broth, SS Agar, Violet Red Bile Agar, Brilliant Green Bile Broth and E.E Broth.
• Bile salt increases the selectivity of the medium by inhibiting gram positive microbes.
• This Bile salt entered in the growth pattern and altered fermentation of lactose.
• It does not allow growing faecal Staphylococci, Pneumococcus and Streptococcus.

PHYSICAL PARAMETERS

 

S.L. No.

PRODUCT PARAMETERS

SPECIFICATION

1.

Appearance

Greenish yellow colour free flowing powder having characteristic odour and smell.

2.

Solubility (2% soln. at 25oC)

Completely soluble in distilled water and alcohol, clear.

3.

Clarity (2% Soln. at 121 ºC.)*

Absolute clear solution. No ppt.

* -After Autoclave

 

CHEMICAL PARAMETERS

 

S.L. No.

PRODUCT PARAMETERS

SPECIFICATION

1.

pH  (2% Soln. at 25 ºC)

6.0 - 8.0

2.

Loss on drying ( at 105 ºC)

NMT - 5.0%

3.

Total Ash

NMT - 20.0%

4.

Cholic Acid

NLT - 45.0%

 

MICROBIAL PARAMETERS

 

S.L. No.

PRODUCT PARAMETERS

SPECIFICATION

1.

Total viable count

NMT- 5,000cfu/gm

2.

Escherichia coli

Negative

3.

Salmonella spp.

Negative

4.

Staphylococcus aureus

Negative

5.

Yeast & molds

50/gm

 

 

 GROWTH PROMOTION TEST

*Prepare a sterile medium with 10 gm peptone, 10 gm lactose, 20 gm Bile salt (STD) TBL & 0.0133 gm brilliant green in 1Liter distilled water. Adjust pH to 7.2 ± 0.2. Inoculate with the bellow mentioned organisms & incubate at 35 – 370C for 48 hrs.

 

S.L. No.

MICROORGANISMS

ATCC

GROWTH

RESULT

1.

Staphylococcus aureus

25923

Inhibited

Complies

2.

Escherichia coli

25922

Good – Luxuriant/ bile ppt.

Complies

3.

Salmonella typhimurium

14028

Good - Luxuriant

Complies

 

Std Packing     : 25kg, HDPE drum

Best Before       : 36 Months

Storage             : Hygroscopic by nature, store the material below 25ºC, protect from direct sunlight.

 
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