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Home » Products » Biotechnology & Fermentation » BIO PEPTONE (STD) TBL POWDER Culture Media Ingredient

BIO PEPTONE (STD) TBL POWDER Culture Media Ingredient

BIO PEPTONE (STD) TBL POWDER is used as the microbiological culture Media Ingredient.

 

PHYSICAL PARAMETERS 

S.L. No.

PRODUCT PARAMETERS

SPECIFICATION

1.

Appearance

Creamish to Yellowish colour, free flowing powder having characteristic odour but not pungent smell.

2.

Solubility (2% Soln. at 25 ºC)

Soluble in distilled Water, Clear. Insoluble in alcohol.

3.

Clarity (2% Soln. at 121 ºC.)*

Absolute clear solution. No ppt.

* -After Autoclave 

CHEMICAL PARAMETERS 

S.L. No.

PRODUCT PARAMETERS

SPECIFICATION

1.

pH  (2% Soln. at 25 ºC)

6.0 - 7.0

2.

Loss on drying (at 105 ºC)

NMT - 6.0%

3.

Total Nitrogen  ( DWB)

NLT - 12.0%

4.

 αAmino Nitrogen

NLT - 3.5%

5.

Total Ash

NMT - 12.0%

6.

Chloride (as NaCl)

NMT - 5.0%

7.

Indole Test

Positive

MICROBIAL PARAMETERS 

S.L. No.

PRODUCT PARAMETERS

SPECIFICATION

1.

Total viable count

NMT- 5,000cfu/gm

2.

Escherichia coli

Negative

3.

Salmonella spp.

Negative

4.

Staphylococcus aureus

Negative

5.

Yeast & molds

50/gm


GROWTH PROMOTION TEST

Cultural Characteristic observed in 2% Bio Peptone (STD) TBL  and 1.5% agar after incubation at 35-37°C for 18-24 hours. 

S.L. No.

MICROORGANISMS

ATCC

Inoculums

(CFU)

GROWTH

RECOVERY

1.

Staphylococcus aureus

25923

50-100

Good-Luxuriant

NLT- 70%

2.

Escherichia coli

25922

50-100

Good-Luxuriant

NLT- 70%

3.

Pseudomonas aeruginosa

27853

50-100

Good-Luxuriant

NLT- 70%

4.

Bacillus subtilis

6633

50-100

Good-Luxuriant

NLT- 70%

5.

Enterococcus faecalis

29212

50-100

Good-Luxuriant

NLT- 70%

6.

Streptococcus pyogenes

19615

50-100

Good-Luxuriant

NLT- 70%

Best Before        : 36 Months

Storage             : Hygroscopic by nature, store the material below 25 ºC, protect from direct sunlight.

 
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