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GELATONE (Gelatin Peptone) Culture Media Ingredient

PHYSICAL PARAMETERS

S.L. No.

PRODUCT PARAMETERS

SPECIFICATION

1.

Appearance

Creamish to light yellowish colour free flowing powder, having characteristic odour but not pungent smell.

2.

Solubility (2% soln. at 25ºC)

Soluble in distilled water, clear.

3.

Clarity (2% soln. at 121ºC)*

Clear solution. No ppt.

        * -After Autoclaving

 

CHEMICAL PARAMETERS

S.L. No.

PRODUCT PARAMETERS

SPECIFICATION

1.

pH  (2% Soln. at 25ºC)

6.5-7.5

2.

Loss on drying (at 105ºC)

NMT- 6.0%

3.

Total Nitrogen  ( DWB)

NLT- 14.0%

4.

α Amino Nitrogen

NLT- 1.5%

5.

Total Ash

NMT- 15.0%

6.

Chloride content (as NaCl )

NMT- 5.0%

 
MICROBIAL PARAMETERS

S.L. No.

PRODUCT PARAMETERS

SPECIFICATION

1.

Total viable count

NMT- 10,000cfu/gm

2.

Escherichia coli

Negative

3.

Salmonella spp.

Negative

4.

Staphylococcus aureus

Negative

5.

Yeast & molds

100/gm

 

GROWTH PROMOTION TEST

Cultural Characteristic observed in 2% Gelatin Peptone  and 1.5% agar after incubation at 35-37°C for 18-24 hours.

S.L. No.

MICROORGANISMS

ATCC

GROWTH

1.

Staphylococcus aureus

25923

Fair to good

2.

Escherichia coli

25922

Good - Luxuriant

3.

Pseudomonas aeruginosa

27853

Good - Luxuriant

4.

Bacillus subtilis

6633

Fair to good

5.

Salmonella typhi

6539

Good - Luxuriant

6.

Streptococcus pyogenes

19615

Good - Luxuriant

 

Std Packing           : 25.0 Kg, HDPE Drum

Best Before     : 36 Months

Storage           : Hygroscopic in nature, store below 25ºC and protect direct sunlight.

 
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