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HEART INFUSION POWDER

PHYSICAL PARAMETERS

S.L. No.

PRODUCT PARAMETERS

SPECIFICATION

1.

Appearance

Light yellow to brownish yellow colour free flowing powder having characteristic odour but not pungent smell.

2.

Solubility (2% soln. at 25ºC)

Soluble in distilled water, clear.

3.

Clarity (2% Soln. at 121ºC.)*

Clear solution. No ppt.

     * -After Autoclave

 

CHEMICAL PARAMETERS

S.L. No.

PRODUCT PARAMETERS

SPECIFICATION

1.

pH (2% soln. at 25ºC)

6.5-7.5

2.

Loss on drying (at 105ºC)

NMT-5.0%

3.

Total Nitrogen Content

NLT- 12.0%

4.

αAmino Nitrogen

NLT- 3.5%

5.

Total Ash

NMT-10.0%

6.

Chloride (as NaCl)

NMT- 6.0%

7.

Indole Test

Positive

 
MICROBIAL PARAMETERS

S.L. No.

PRODUCT PARAMETERS

SPECIFICATION

1.

Total viable count

NMT-10,000cfu/gm

2.

Escherichia coli

Negative

3.

Salmonella spp.

Negative

4.

Staphylococcus aureus

Negative

5.

Yeast & molds

100/gm

 

GROWTH PROMOTION TEST

Cultural Characteristic observed in 2%  Heart Infusion Powder  and 1.5% agar after incubation at 35-37°C for 18-24 hours.

S.L. No.

MICROORGANISMS

ATCC

GROWTH

1.

Staphylococcus aureus

25923

Good - Luxuriant

2.

Escherichia coli

25922

Good - Luxuriant

3.

Pseudomonas aeruginosa

27853

Good - Luxuriant

4.

Bacillus subtilis

6633

Good - Luxuriant

5.

Salmonella typhi

6539

Good - Luxuriant

6.

Streptococcus pyogenes

19615

Good - Luxuriant

 

Std Packing           : 25.0 Kg, HDPE Drum

Best Before     : 36 Months

Storage           : Hygroscopic in nature, store below 25ºC and protect direct sunlight.

 
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